Key features and details | |
Cat. No. | MABL-1376 |
Name | Anti-desmoglein-3 mAbs |
Clone No. | AFD- 5G11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | dot blot, IP, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes desmoglein 3. It does not cross-react with desmoglein 1 or desmoglein 2. Desmoglein-3 is a calcium binding membrane protein that is localized in desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points. |
Alternative Name | PVA; Cadherin family member 6; desmoglein 3; 130 kDa pemphigus vulgaris antigen; DSG3 |
UniProt | P32926 |
Immunogen | This antibody was raised by immunizing Balb/c mice with a recombinant protein corresponding to human desmoglein-3. |
Application Notes | This antibody can be used for detection and analysis of desmoglein-3 via various methods. For instance,5G11 was utilized todetect desmoglein-3 in human scalp hair follicles and epidermal inclusions via immunohistochemistry (Wu et al., 2003). Another group successfully used this antibody to identify desmoglein-3 via Western blot (Mao et al., 2017; pmid: 28469076). The antibody 5G11 was also used to detect DSG3 in cells via immunofluorescence (Chen et al., 2013; pmid: 23737966). |
Antibody First Published | Wahl Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics. 2002 Feb;21(1):37-44. PMID: 11991815 |
Note on publication | This article describes the generation and characterization of the antibody 5G11. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
