Key features and details | |
Cat. No. | MABL-1381 |
Name | Anti-Diethoxyphosphorothioate mAbs |
Clone No. | AFD- 12C2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure. |
Alternative Name | O,O-diethyl organophosphorus pesticides; DOPs; OPs |
UniProt | |
Immunogen | The antibody was generated by immunizing BABL/c mice with the diethoxyphosphorothioate derivated hapten, followed by antibody library construction and phage display-based selection. |
Application Notes | The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787). The specificity of the original format of the antibody was confirmed by competitive indirect ELISA analysis (Xu et al. 2010; PMID: 20958019). The original format of the antibody was linked to alkaline phosphatase (scFv−AP) and employed to develop a competitive direct enzyme-linked immunosorbent assay (cdELISA) for O,O-diethyl OPs in environmental and agricultural samples (Xu et al. 2012; PMID: 22533788). |
Antibody First Published | Chen et al. Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition. Int J Mol Sci. 2018 May; 19(5): 1381. PMID: 29734787 |
Note on publication | The paper describes the generation and characterization of the Fab fragment. The antibody reacts against a broad set of O,O-diethyl organophosphorus pesticides (DOPs). The 3D model of Fab was built via homologous modeling and the interaction between Fab and DOPs was studied via molecular docking and dynamics simulations. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
