Key features and details | |
Cat. No. | MABL-1399 |
Name | Anti-Dll1 mAbs |
Clone No. | AFD- HMD1-5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Functional Studies, FC, IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | The antibody HMD1-5 recognizes Dll1 which is a one of five type I transmembrane proteins that serves as a Notch receptor ligand. |
Alternative Name | Delta1; Delta-like protein 1; Drosophila Delta homolog 1 |
UniProt | Q61483 |
Immunogen | The original hamster IgG version of this antibody was raised by immunizing Armenian hamsters (Oriental Yeast) with Dll1-expressing CHO cells via intraperitoneal injections and subsequent generation of the hybridoma cells. |
Application Notes | This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The original hamster IgG version of this antibody was successfully used as a blocking agent to inhibit the binding of Notch2-Fc to Dll1/CHO cells (Moriyama et al., 2008; pmid: 18381350), demonstrating that this clone is an effective inhibitor of the interactions between Dll1 and Notch receptors. Another study investigated the role of Delta1 in allograft rejection by blocking it with HMD1-5 (Riella et al., 2011; pmid: 21949024). This blocking ability was further corroborated by showing that HMD1-5 stops the Dll1-Fc-triggered Notch2 signaling (Moriyama et al., 2008; pmid: 18381350). |
Antibody First Published | Moriyama et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice Int Immunol. 2008 Jun;20(6):763-73. doi: 10.1093/intimm/dxn034. Epub 2008 Apr 1. PMID: 18381350 |
Note on publication | Describes the generation and characterization of the HMD1-5 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
