Anti-DNA-RNA hybrid mAbs

Cat. No.

MABL-1414

Name

Anti-DNA-RNA hybrid mAbs

Clone No.

AFD- S9.6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ABA, CHIP, ChIP-seq, DB, EMSA, FISH, ICC,   IP, PLA, SPR, IF

Species Reactivity

Species independent

Basic Information

Specificity

This antibody demonstrates high specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids, making it an effective tool for detecting R-loops. It does not cross-react with single- stranded DNA, double-stranded DNA, RNA, or ribosomal RNA. In the original study by Boguslawski et al. (1986), the antibody was shown to bind DNA–RNA heteropolymers and poly(I)–poly(dC) with equal affinity, though 100-fold higher concentrations of poly(A)–poly(dT) were required for comparable binding. DNA–RNA hybrids naturally occur in eukaryotic cells,   particularly at sites of high transcriptional activity. These non- canonical   nucleic acid structures have transcriptional regulatory functions and are  associated with genomic instability. The presence of DNA–RNA hybrids can   predispose specific loci to chromosomal breakage. 

Alternative Name

DNA:RNA Duplex; DNA-RNA Duplex; DNA/RNA Duplex; DNA-RNA Hybrid; DNA/RNA Hybrid; DNA:RNA Hybrid; RNA-DNA Duplex; RNA/DNA Duplex; RNA:DNA Duplex; RNA-DNA Hybrid; RNA/DNA Hybrid; RNA:DNA   Hybrid

UniProt

Immunogen

This antibody was generated in BALB/c mouse   against S9.6 ΦX174 bacteriophage-derived synthetic DNA/RNA antigen according   to the protocol by Boguslawski et al. (J. Immunol Methods. 1986).

Application Notes

DNA–RNA hybrids naturally occur in   eukaryotic cells, with their levels increasing in regions of high   transcriptional activity, such as during transcription initiation,   repression, and elongation. These hybrids play a crucial role in influencing   genomic instability, making the anti-DNA–RNA hybrid (S9.6) antibody an   invaluable tool for studying R-loops and hybrid-induced lesions during DNA   replication and other cellular processes. This antibody also excels in   recognizing RNA–DNA hybridization in microarray studies. Key applications of   the S9.6 antibody include affinity binding assays (ABA), chromatin   immunoprecipitation (ChIP), chromatin immunoprecipitation-sequencing   (ChIP-seq), dot blot (DB), fluorescent in situ hybridization (FISH),   electrophoretic mobility shift assays (EMSA), surface plasmon resonance   (SPR), immunofluorescence (IF), immunocytochemistry (ICC), and immunoprecipitation (IP). 

Antibody First Published

Boguslawski et al. Characterization of   monoclonal antibody to DNA.RNA and its application to immunodetection of   hybrids. J Immunol Methods. 1986 May 1;89(1):123-30. PMID:2422282

Note on publication

The original publication describes the   generation of this antibody, its characterization, and its application to the   immunodetection of DNA-RNA heteropolymer hybrids.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.