Key features and details | |
Cat. No. | MABL-1419 |
Name | Anti-Do (b) mAbs |
Clone No. | AFD- Mima-73 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for Dombrock b, a blood group antigen carried by ART4. Haemagglutination studies have shown this antibody to react with RBCs from non-human primates, including chimpanzee and orangutan (Mogos et al, 2003). |
Alternative Name | CD297; Dob; Dombrock glycoprotein; Dombrock (b); 15D6; ADP- ribosyltransferase 4; Ecto-ADP-ribosyltransferase 4; ART4; ARTC4; DO; DO/ART4; DOK1 |
UniProt | Q93070 |
Immunogen | This antibody was raised by immunising BALB/c mice with human embryonic kidney 293T cells transfected to express high levels of Dombrock b. |
Application Notes | The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012). This antibody has been used, as part of a panel of antibodies specific for different epitopes on the Dombrock glycoprotein, in flow cytometric and haemagglutination analysis of human red blood cells (Mayer et al, 2010; Costa et al, 2010). |
Antibody First Published | Grodecka et al. The production, serologic evaluation, and epitope mapping of ten murine monoclonal Dombrock antibodies Immunohematology. 2012;28(4):124-9. PMID:23421541 |
Note on publication | Describes the original generation of this antibody, and the characterisation of its antigen specificity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
