Key features and details | |
Cat. No. | MABL-1422 |
Name | Anti-domoic acid mAbs |
Clone No. | AFD- DA24cB7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Specie independent |
Basic Information | |
Specificity | The antibody is specific for domoic acid. Domoic acid is a kainic acid-type neurotoxin that causes amnesic shellfish poisoning. It is produced by algae and accumulates in shellfish, sardines, and anchovies. When sea lions, otters, cetaceans, humans, and other predators eat contaminated animals, poisoning may result. |
Alternative Name | DA; (2S,3S,4S)-4-[(2Z,4E,6R)-6-Carboxyhepta-2,4-dien-2-yl]-3- (carboxymethyl)pyrrolidine-2-carboxylic acid |
UniProt | |
Immunogen | The original antibody was originated from a scFv phage library obtained from a sheep hyperimmunised with DA-BSA conjugate. It was isolated by panning against DA. |
Application Notes | The specificity of the original format of the antibody was confirmed by competitive ELISA using plates coated with DA-OVA (I50 of 1.21 ng/mL (3.9 nM)). The assay was used for the determination of DA levels in shellfish extracts (Shaw et al., 2008; PMID: 18384202). |
Antibody First Published | Shaw et al. Development of a high-affinity anti-domoic acid sheep scFv and its use in detection of the toxin in shellfish Anal Chem. 2008 May 1;80(9):3205-12. doi: 10.1021/ac7024199. Epub 2008 Apr 3. PMID:18384202 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
