Anti-Dxd mAbs

Cat. No.

MABL-1444

Name

Anti-Dxd mAbs

Clone No.

AFD- 12G9-2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Species independent

Basic Information

Specificity

This antibody binds Dxd, an anti-cancer   alkaloid camptothecin analog, which is a potent DNA topoisomerase I (TOP1)   inhibitor. By forming a stable ternary complex with TOP1 and DNA, it arrests   the cell cycle to S phase, thereby preventing DNA replication and causing   cell death. DxD is used for the generation of antibody-drug conjugates for   targeted delivery of toxic materials to cancerous cells. A HER2 targeting -   DxD drug conjugate (DS-8201a) shows potent antitumor activity in   HER2-positive models

Alternative Name

Exatecan derivative; Camptothecin analog

UniProt

Immunogen

The original antibody was generated by   immunizing BALB/c mice with Dxd-KLH conjugate.

Application Notes

The binding reactivity of this antibody   towards Dxd-KLH and Dxd-BSA conjugates was determined using ELISA. This   antibody is capable of binding antibody-drug conjugates ADC-8201 and ADC029   in a competitive ELISA, wherein the antibody was trastuzumab. The binding  affinity of this antibody towards Dxd-BSA was determined using surface   plasmon resonance (SPR) and it was reported to be Kd= 3.38nM (CN115677857).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.