Anti-Dynein Light Chain Tctex-Type 3 (DYNLT3) mAbs

Cat. No.

MABL-1447

Name

Anti-Dynein Light Chain Tctex-Type 3 (DYNLT3) mAbs

Clone No.

AFD- 10A3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for Dynein Light   Chain Tctex-Type 3 (DYNLT1). Dynein Light Chain Tctex-Type 3 acts as one of   several non-catalytic accessory components of the cytoplasmic dynein 1   complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as   a motor for the intracellular retrograde motility of vesicles and organelles   along microtubules. Probably binds BUB3 as part of transport cargo. Required   for the efficient progression through mitosis (By similarity).

Alternative Name

DYNLT3; Protein 91/23; T-complex-associated testis-expressed 1-like

UniProt

P51808

Immunogen

The light chain-myosin basic protein fusion protein   was expressed in bacteria and the purified fusion protein was injected into   mice to generate the monoclonal antibody.

Application Notes

The antibody has been used for   immunofluorescence on PC12 cell neurites (Ha et al, 2008; pmid:18559670).   Western blot has been performed on PC12 cells using this antibody (Ha et al,   2008; pmid:18559670). It was used for immunoprecipitation on the bacteria   expressing the GST fusion proteins (Lo et al, 2007; pmid:17965411). The   antibody was used for immunoblot assays on bacteria expressing the GST fusion   proteins as well (Lo et al, 2007; pmid:17965411). Mitotic NRK cell lysate has   been used for immunoprecipitation with this antibody (Lo et al, 2007;   pmid:17289665). Immunoblotting was preformed on Mitotic NRK cell lysate with   this antibody (Lo et al, 2007; pmid:17289665). PC12 cell lines were used for   a western blot with this antibody (Myers et al, 2007; pmid:17279546).  

Antibody First Published

Lo et al. Interaction of the DYNLT   (TCTEX1/RP3) Light Chains and the Intermediate Chains Reveals Novel   Intersubunit Regulation during Assembly of the Dynein Complex J Biol Chem.   2007 Dec 21;282(51):36871-8. PMID:17965411

Note on publication

It describes the generation of this antibody   from fusion proteins.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.