Key features and details | |
Cat. No. | MABL-1456 |
Name | Anti-E-selectin mAbs |
Clone No. | AFD- 7A9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognizes the endothelial leukocyte adhesion molecule-1 (ELAM-1) protein as expressed in COS cells. |
Alternative Name | CD62E; ELAM-1; ELAM1; Leukocyte-endothelial cell adhesion molecule 2; endothelial leukocyte adhesion molecule-1; CD62 antigen-like family member E; Leukocyte-endothelial cell adhesion molecule 2; SELE |
UniProt | P16581 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with IL-1 activated human endothelial cells. |
Application Notes | The F(ab')2 fragment of this antibody showed dose-dependent inhibition of cells activated with IL-1 beta, cells used were human granulocytes. The antibody binds to IL-1 activated human endothelial cells (HEC) but not significantly to normal resting HEC. |
Antibody First Published | Graber et al. T cells bind to cytokine-activated endothelial cells via a novel, inducible sialoglycoprotein and endothelial leukocyte adhesion molecule-1. J Immunol. 1990 Aug 1;145(3):819-30. PMID:1695646 |
Note on publication | A study on effect of human rIL-1 beta on confluent, quiescent monolayers of human umbilical vein endothelial cells (HUVEC) was performed. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
