Key features and details | |
Cat. No. | MABL-1467 |
Name | Anti-EDIII of DENV2 mAbs |
Clone No. | AFD- 3H5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vivo, neutralizing, SPR, ELISA, FC |
Species Reactivity | Dengue Virus |
Basic Information | |
Specificity | The antibody binds to domain III of the envelope (E) protein of Dengue virus. In particular, it targets residues buried between E dimers and located close to the viral membrane. The antibody is serotype specific to DENV2. The E protein is a structural protein located on the viral surface and consists of three distinct domains termed EDI, EDII, and EDIII. Of these, EDIII mediates attachment to target cells, prior to internalization into host endosomes. |
Alternative Name | E-protein; DENV EDIII; Dengue envelope protein domain III; E protein domain III; EDIII; DVC-21; DV 21; DV-21; Envelope protein E |
UniProt | W5RZ25 |
Immunogen | The original antibody was generated by immunizing mice with DENV2 strain New Guinea C (NGC). |
Application Notes | Binding avidity/affinity of both Full length (IgG) and Fab fragments of the antibody to whole virion measured by ELISA. The crystal structure of the Fab fragment in complex with EDIII of DENV2 NGC was determined. Focus reduction neutralization assays showed highly potent neutralization of DENV2 NGC and 16681 for the IgG1 fragment with 50% neutralization titers in the sub-nanomolar range (50% FRNT at 0.044 nM ). Pre- and postattachment neutralization assays showed the antibody is capable of significantly inhibiting infection after the virus had bound to the cells. The antibody promotes little antibody dependent enhancement (ADE). ADE was analyzed using U937 cells, the antibody showed no enhancing capacity for DENV2 NGC and reduced enhancement of DENV2 16681 at a very narrow concentration range. Also the IgG2a format of the antibody showed no enhancement of infection for DENV2 NGC while still potently neutralizing infection. |
Antibody First Published | Gentry et al. Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. Am. J. Trop. Med. Hyg. 31:548– 555. PMID:6177259 |
Note on publication | The paper describes the generation and characterization of the antibody, which is specific for Dengue virus. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
