Key features and details | |
Cat. No. | MABL-1483 |
Name | Anti-EME1 mAbs |
Clone No. | AFD- 7h2/1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises human EME1. EME1 together with MUS81 create a DNA endonuclease which binds preferentially to branched DNA structures such as Holliday junctions and replication forks. It's engaged in processing of stalled or collapsed replication forks in mitosis. |
Alternative Name | Essential meiotic endonuclease 1; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4; EC 3.1.22; EC 3.1.22.; essential meiotic endonuclease 1 homolog 1 (S. pombe); essential meiotic endonuclease 1 homolog 2; FLJ31364; homolog of yeast EME1 endonuclease; MMS4L; MMS4; SLX2 structure-specific endonuclease subunit homolog A; SLX2A; MTA31 |
UniProt | Q96AY2 |
Immunogen | The antibody was raised against His-tagged recombinant Eme1 of human origin. |
Application Notes | WB: MTA31 7h2/1 antibody was used to detect EME1 in western blot analysis in the experiments assessing EME1 as a potential marker of cisplatin resistance in cancer therapy (Tomoda et al., 2009). WB: This antibody was used to detect EME1 on western blots after previous immunopurification procedure (Laguette et al., 2014). IHC: Other group conducted an immunohistochemistry experiment staining against EME1 in formalin-fixed paraffin embedded esophageal adenocarcinoma tissue samples using MTA31 7h2/1 antibody (Dwalt et al., 2014). |
Antibody First Published | Tomoda et al. Functional evidence for Eme1 as a marker of cisplatin resistance. Int J Cancer. 2009 Jun 15;124(12):2997-3001. doi: 10.1002/ijc.24268. PMID:19267403 |
Note on publication | This article describes the earliest found instance of application of MTA31 7h2/1 antibody which was used to detect EME1 in western blot analysis in the experiments assessing EME1 as a potential marker of cisplatin resistance in cancer therapy. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
