Key features and details | |
Cat. No. | MABL-1562 |
Name | Anti-FAP mAbs |
Clone No. | AFD-73.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, IP, WB, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs). |
Alternative Name | Fibroblast activation protein; Fibroblast activation protein-α; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha1; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme;Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase |
UniProt | P97321 |
Immunogen | The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells. |
Application Notes | A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4–1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35–50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). |
Antibody First Published | Wang et al. Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicity Cancer Immunol Res. 2014 Feb;2(2):154-66. PMID:24778279 |
Note on publication | The paper describes the generation of the antibody and the generation of the anti-muFAP-CAR construct. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
