Anti-FAS mAbs

Cat. No.

MABL-1564

Name

Anti-FAS mAbs

Clone No.

AFD- HFE7A

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, in vitro, in vivo, ELISA, FC

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody is specific for human and   mouse FAS.

Alternative Name

CD95; APT1; FAS1; TNFRSF6; Apo-1 antigen;   FASLG receptor; Apoptosis- mediating surface antigen FAS; Tumor necrosis   factor receptor superfamily member 6

UniProt

P25445; P25446

Immunogen

The original antibody was raised by   immunizing a Fas-deficient mice with partially purified human Fas antigen.

Application Notes

The original antibody was used for flow   cytometry on activated T cells from humans, chimpanzees, Japanese monkeys,   crab-eating monkeys and marmosets, and on thymocytes from BALB/c mice. Here   it was determined that this antibody was reactive to humans and mice. This   antibody was used for a cytotoxic assay on synovial cells (Ichikawa et al.,   2000; PMID:10744657). The structure of the original antibody was determined   using x-ray crystallography (Ito et al., 2002; PMID:11754745, Haruyama et   al., 2002; PMID:12499636). This antibody was used for a competitive ELISA on   Fas (Haruyama et al., 2002; PMID:12499636, Yoshida et al., 2010;   PMID:20024619). This antibody was used for surface plasmon resonance on mouse   Fas, to determine binding affinity. The protective effects of this antibody   against acute and lethal hepatic injury induced by Jo2 was assessed. It   showed it was able to protect against hepatic injury. To assess the effect of   this antibody on caspases in vivo, mice were injected with the antibody after   Jo2-induced apoptosis of hepatocytes. It showed that it strongly inhibited   activation of caspases (Yoshida et al., 2010; PMID:20024619).

Antibody First   Published

Ichikawa et al. A novel murine anti-human   Fas mAb which mitigates lymphadenopathy without hepatotoxicity Int Immunol.   2000 Apr;12(4):555-62. PMID:10744657

Note on publication

In the original paper they report on the   interesting characteristics of the newly obtained anti-Fas mAb, HFE7A, which   cross-reacts with the Fas molecules of various species ranging from human to   mouse and mitigates autoimmune symptoms without hepatotoxicity in mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.