Key features and details | |
Cat. No. | MABL-1611 |
Name | Anti-FlhA mAbs |
Clone No. | AFD-1199 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Dot Blots, ELISA |
Species Reactivity | Ca. Liberibacter asiaticus |
Basic Information | |
Specificity | The antibody binds the flha protein of Ca. Liberibacterasiaticus. |
Alternative Name | Flagellar biosynthesis protein FlhA |
UniProt | C6XF33 |
Immunogen | The original antibody was generated by panning against a naturally expressed murine library. |
Application Notes | Candidatus Liberibacter asiaticus (CaLas) is the pathogen that causes citrus Huanglongbing (HLB), the most serious disease of citrus. The paper describes the development of single chain variable fragment (scFv) antibodies against surface proteins of ‘Ca. Liberibacter asiaticus’ as potential diagnostic methods. A primary library of recombinant single chain variable fragment antibody was developed and the library was screened for antibodies that bind to specifically-chosen proteins that are present on the surface of CaLas. In particular, dot blot identified FlhA as a protein which is expressed on the surface of CaLas and ELISA result showed the antibody 1199 binds Flha (A450=0.26) (PMID 26744234). |
Antibody First Published | Yuan et al. Development of single chain variable fragment (scFv) antibodies against surface proteins of 'Ca. Liberibacter asiaticus'. J Microbiol Methods. 2016 Mar;122:1-7. PMID:26744234 |
Note on publication | Development of a library of recombinant single chain variable fragment (scFv) antibodies which could be used as a diagnostic method for huanglongbing. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
