Key features and details | |
Cat. No. | MABL-1662 |
Name | Anti-GD2 mAbs |
Clone No. | AFD- 3F8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, ICC, in vitro, in vivo, SPR, therapeutic, TLC, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human disialoganglioside GD2, a surface antigen highly expressed on human neuroblastoma cells. The epitope is located at the putative membrane interaction site. This antibody shows strong binding to NB cells and no cross-reactivity to normal human tissues except for human cortical neurons. Furthermore, it exhibits no cross-reactivity to most other gangliosides of those tested (GM2, GD1a, GT1b, GD3, and GQ1b) except GD1b to which it had very low cross-reactivity. |
Alternative Name | disialoganglioside GD2 |
UniProt | Q9UI17 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human neuroblastoma cells. |
Application Notes | The original version of this antibody (mouse IgG3) was first shown to specifically bind human GD2 via ICC (Saito et al., 1985; PMID: 2579648). The antibody was used in indirect IF assays to detect the GD2 ganglioside on the surface of neuroblastoma cells and applied in ELISA to screen and quantify GD2 binding. Inhibition ass ays were conducted to assess its specificity for GD2 over other glycolipids. In thin-layer chromatography (TLC) coupled with immunostaining, the antibody was used to identify and confirm the presence of GD2 ganglioside in cultured neuroblastoma cell extracts. Additionally, complement-mediated cytotoxicity (CMC) assays demonstrated its ability to induce immune-mediated cell death in GD2-expressing neuroblastoma cells, successfully killing 100% of these cells while sparing normal bone marrow cells (Cheung et al., 1985; PMID: 2580625). The antibody was also tested in vivo in a phase I clinical trial, where it mediated antibody-dependent cellular cytotoxicity (ADCC) and complement activation without crossing the blood-brain barrier (Cheung et al., 1987; PMID: 3625258). |
Antibody First Published | Cheung et al., Monoclonal antibodies to a glycolipid antigen on human neuroblastoma cells. Cancer Res. 1985 Jun;45(6):2642-9. PMID:2580625 |
Note on publication | The original publication describes the development and characterization of murine monoclonal antibodies that specifically target a glycolipid antigen on human neuroblastoma cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
