Key features and details | |
Cat. No. | MABL-1668 |
Name | Anti-Gelsolin mAbs |
Clone No. | AFD- GsnVHH 11 (Nb11) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunotherapy, IP, WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes gelsolin irrespective of calcium and completely blocks its interaction with G-actin. This antibody specifically recognizies and epitope in the G2-G3 domain of gelsolin. |
Alternative Name | GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin |
UniProt | P06396 |
Immunogen | The original antibody was generated by immunizing a llama subcutaneously with human recombinant gelsolin. |
Application Notes | Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds gelsolin with a Kd of ~5 nM irrespective of calcium and completely blocks its interaction with G-actin. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. It was also reported that free gelsolin is targeted to the nucleus by nuclear localizationsequence (NLS)-GsnVHH 11 in HeLa cells (PMID:20140750). |
Antibody First Published | Abbeele et al. A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction. Cell Mol Life Sci. 2010 May;67(9):1519-35. PMID:20140750 |
Note on publication | Describes the generation, characterization, epitope mapping and crystal structure of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
