Key features and details | |
Cat. No. | MABL-1691 |
Name | Anti-Ghrelin mAbs |
Clone No. | AFD-1621 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | calcium assay, SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody has specificity for both the acylated and unacylated form of ghrelin. |
Alternative Name | Appetite-regulating hormone; growth hormone secretagogue; protein M46 |
UniProt | Q9UBU3 |
Immunogen | Mice were immunised with human ghrelin acylated at the His residue position 9. |
Application Notes | ELISA demonstrated that 1621 binds full length human ghrelin independent of its acylation status, and binds poorly to rat ghrelin. The in vitro FLIPR Calcium Assay system was used to show that 1621 binds residues 4-20 of human ghrelin. Using SPR, the Kd of 1621 binding to acylated human ghrelin was determined as 6.80 x 10^-10 M; the Kd of 162 binding to deacylated human ghrelin was determined as 1.74 x 10^-9 M. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
