Key features and details | |
Cat. No. | MABL-1710 |
Name | Anti-Glycophorin A mAbs |
Clone No. | AFD- 10F7MN |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognizes the N and M-type of glycophorin A. |
Alternative Name | CD235a; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; Glycophorin A, type M and type N |
UniProt | P02724 |
Immunogen | The antibody was generated by immunizing mice with Glycophorin A. |
Application Notes | The Fab fragment recognized glycophorin a on the surface of erythrocytes by immunoprecipitation or western blotting techniques. The number of binding sites and dissociation constants of the Fab' fragment was determined (n = 4.66 × 10^5 sites/erythrocyte, Kd =9.5 × 10^-8 M) (Catimel et al. 1993; PMID： 8228269). This antibody was used for detection of Glycophorin A by flow cytometry (Lansdorp et al., 1992; PMID： 1375263). |
Antibody First Published | Bigbee et al. Monoclonal antibodies specific for the M- and N-forms of human glycophorin A Mol Immunol. 1983 Dec;20(12)：1353-62. PMID：6197636 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
