Key features and details | |
Cat. No. | MABL-1718 |
Name | Anti-Glycoprotein GPIIb/IIIa complex mAbs |
Clone No. | AFD-314.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vitro, in vivo, SPR, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognizes the N terminus of the GPIIb beta propeller domain only when soluble quinine is present. |
Alternative Name | Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61 |
UniProt | P05106 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a mixture of quinine- linked GPIIb/IIIa, unlinked GPIIb/IIIa, and soluble quinine. |
Application Notes | The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry. Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). Nonobese diabetic/severe combined immunodeficient (NOD/scid) mouse was used as a model to study drug-dependent clearance of platelets in vivo. Mice were first infused with human platelets and then injected with the antibody and quinine or buffer; platelets were cleared from the circulation when the antibody and quinine were injected, whereas the antibody alone was without effect. (Bougie et al., 2010; PMID: 20587782) GPIIb/IIIa bound monovalently to the immobilized antibody with low affinity in the absence of quinine and with fivefold greater affinity when quinine was present (KD ≈ 2.2*10^- 6), as shown by SPR analysis. |
Antibody First Published | Bougie et al. Quinine-dependent, platelet-reactive monoclonals mimic antibodies found in patients with quinine-induced immune thrombocytopenia. Blood. 2009 Jan 29; 113(5): 1105–1111. PMID:18948570 |
Note on publication | The paper describes the generation and characterization of the antibody. The antibody is employed in studies to define the molecular basis for drug-dependent antibody binding and platelet destruction in DITP. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
