Key features and details | |
Cat. No. | MABL-1734 |
Name | Anti-GP25-200 mAbs |
Clone No. | AFD- 4H9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, WB, ELISA |
Species Reactivity | Cryptosporidium parvum |
Basic Information | |
Specificity | This antibody binds the GP25-200 antigen of Cryptosporidium parvum. Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences. |
Alternative Name | GP25-200 antigen |
UniProt | |
Immunogen | The original antibody was generated by immunizing female BALB/c mice intraperitoneally and subcutaneously with purified P23 emulsified in monophosphoryl A-trehalose dimycolate adjuvant. |
Application Notes | This antibody can be used for the identification of sporozoites and GP25-200 antigen of C. parvum using western blot. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143). |
Antibody First Published | Schaefer et al. Characterization and formulation of multiple epitope-specific neutralizing monoclonal antibodies for passive immunization against cryptosporidiosis. Infect Immun. 2000 May;68(5):2608-16. PMID:10768951 |
Note on publication | Describes the generation and characterization of antibodies against multiple epitope of C. parvum to provide passive immunization against Cryptosporidiosis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
