Anti-H-2Kk MHC mAbs

Cat. No.

MABL-1755

Name

Anti-H-2Kk MHC mAbs

Clone No.

AFD- AF3-12.1.3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for the mouse H-2Kk MHC antigen. H-2Kk MHC is involved in the presentation of foreign   antigens to the immune system.

Alternative Name

H-2 class I histocompatibility antigen, K-K   alpha chain; H2-K1; H-2K(K); H2-K

UniProt

P04223

Immunogen

The antibody was raised against the mouse   H-2Kk MHC antigen.

Application Notes

The passive transfer of monoclonal   antibodies was tested using this antibody. This experiment was preformed on   cardiac allograft recipients (Rahimi et al, 2004; pmid:14961984). It was   tested whether this antibody could activate the complement. It could not   (Takenaka et al, 2012; pmid:22980951). Bone marrow plug grafts were removed   from the kidney capsule and used for flow cytometry using this antibody. The   test animal used was a mouse (Dai et al, 2017; pmid:28783664). Mouse   single-cell suspensions from the thymus, lymph nodes, and spleen were used   for flow cytometry with this antibody (Martínez-Riaño et al, 2019;   pmid:31324740).

Antibody First   Published

Rahimi et al. Non-complement- and   complement-activating antibodies synergize to cause rejection of cardiac  allografts Am J Transplant. 2004 Mar;4(3):326-34. PMID:14961984

Note on publication

Alloantibodies (AlloAbs) are a clinically   significant component of the immune response to organ transplants. The effect   of these antibodies was tested on mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.