Key features and details | |
Cat. No. | MABL-1771 |
Name | Anti-HA tag mAbs |
Clone No. | AFD- 12CA5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for the HA-tag. HA contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity. |
Alternative Name | Rho GTPase-activating protein 45; Arhgap45; Minor histocompatibility protein HA-1 |
UniProt | Q3TBD2 |
Immunogen | This antibody was raised by immunizing balb/c mice with a 9 amino acid petide to influenza virus hemagglutinin. |
Application Notes | The mouse version of this antibody has shown the ability to immunoprecipitate HA bound to BAX from cell lysates (Cheng et al, 1996; pmid:8596636). HA tagged SIM1 was immoprecipitated using the mouse version of this antibody. Other proteins present that lacked the HA tag were not immunoprecipitated (Michaud et al, 2000; pmid:10640708). A western blot has detected HA bound to PI4P5K using the mouse version of this antibody (Shibasaki et al, 1997; pmid:9065410). The HOXC6 protein bound to a HA-tag was immunoprecipitated using the mouse version of this antibody (McCabe et al, 2008; pmid:18339881). Cells expressing MLS2 fused to the HA epitope were stained using the mouse version of this antibody. Further the MSL2-HA protein was immuoprecipitated using this antibody (Smith et al, 2000; pmid:10594033). The Pat1 gene tagged with HA can be immunoprecipitated from lysed yeast cells using the mouse version of this antibody. |
Antibody First Published | Cheng et al. Bax-independent inhibition of apoptosis by Bcl-XL Nature. 1996 Feb 8;379(6565):554-6. PMID:8596636 |
Note on publication | Using site-specific mutagenesis,it is shown here that the amino-acid residues critical for protection of cells by Bcl-XL against Sindbis virus-induced apoptosis are clustered within the Bcl-2- homology regions 1 and 2 (BH1 and BH2 regions). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
