Key features and details | |
Cat. No. | MABL-1795 |
Name | Anti-Hemagglutinin 5 mAbs |
Clone No. | AFD- H5.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize |
Species Reactivity | Anhui, HK/156, Indo), Influenza virus H5N1 (VN/1203) |
Basic Information | |
Specificity | H5.3 binds to H5 at the head domain at an epitope which involves the 190 loop. The epitope was determined to be LGVSSA (aa 134-139) and YQGKSS (aa 142-147) of the head domain by DXMS (deuterium exchange mass spectrometry). This antibody shows reactivity with the wild-type H5N1 field strains VN/1203 (EC50 ~ 17 ng/ml), HK/156 (EC50 ~ 18 ng/ml), Anhui (EC50 ~ 2.8 ug/ml) and Indo (EC50 ~ 10 ug/ml). HA is a viral glycoprotein initially assembled as a trimer on the viral envelope as the preprotein HA0. It is subsequently cleaved by host proteases. |
Alternative Name | H5 HA |
UniProt | Q5EP31 |
Immunogen | H5.3 was generated from hybridomas created by fusing PBMCs (peripheral blood mononuclear cells) from human donors who had been previously received an A/Vietnam/1203/2004 H5N1 subunit vaccine with myeloma cells. The hybridoma cells were screened for those showing binding affinity for H5 HA. |
Application Notes | H5.3 binds to H5 where it is able to block the receptor binding site on HA by inserting its CDR3 into the site (IC50 ~ 20 ng/ml). H5.3 can neutralize wild-type VN/1203, rdt VN/1203 K and VN/1203 F pseudovirions, all with IC50s of 5 ng/ml. This antibody also has potent HI (hemagglutination inhibition) activity (80 ng/ml). |
Antibody First Published | Thornburg et al. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses. J Clin Invest. 2013 Oct;123(10):4405-9. PMID:23999429 |
Note on publication | Describes the generation of anti-H5 HA antibodies. Their binding and neutralizing activities were studied in vitro. The structure of H5.3 was determined by x-ray crystallography and a low- resolution structure of H5.3 complexed with VN/1203 HA trimer was determined by EM. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
