Key features and details | |
Cat. No. | MABL-1797 |
Name | Anti-Hemoglobin monomer 14kDa mAbs |
Clone No. | AFD- 13F7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Cow, Horse, Mammals, Pig, Sheep |
Basic Information | |
Specificity | 13F7 recognizes a hemoglobin monomer (14 Da) in blood samples of various mammals (pig, sheep, horse, cow). |
Alternative Name | haemoglobin monomer; Hb; Hbg; PHb; mAb13F7 |
UniProt | |
Immunogen | The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a mammalian blood immunogen. |
Application Notes | This antibody is recommended for the identification of hemoglobin and, more generally, blood in various samples and products, such as commercial meat products. It was used in the development of a successful ELISA-based assay for the quantification of hemoglobin (PHb) in meat products. It had a very wide working range from 0.5 ppm to 1000 pp, high sensitivity and precision (Jiang et al., 2018; pmid: 29412908). Testing by Western blot demonstrated that 13F7 is able to detect a hemoglobin monomer (mass: 14 Da) in blood samples of different mammals (Jiang et al., 2018; pmid: 29412908). Consequently, one of the potential areas where this antibody will be particularly useful is the food industry and food control against adulteration with meat products. |
Antibody First Published | Jiang et al. Monoclonal antibody-based ELISA for the quantification of porcine hemoglobin in meat products Food Chem. 2018 Jun 1;250:170-179. doi: 10.1016/j.foodchem.2018.01.032. Epub 2018 Jan 6. PMID:29412908 |
Note on publication | Describes the generation and characterization of the antibody 13F7 as well as the construction of an assay detecting blood in food products. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
