Key features and details | |
Cat. No. | MABL-1799 |
Name | Anti-hemorrhagic protein mAbs |
Clone No. | AFD-H9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, ELISA |
Species Reactivity | Bothrops atrox |
Basic Information | |
Specificity | The original antibody is specific for the hemorrhagic protein of Bothrops atrox. |
Alternative Name | Bap-1; SVMP; Snake venom metalloproteinase BaP1; Hemorrhagic metalloproteinase BaP1 |
UniProt | P83512 |
Immunogen | The original antibody was generated by immunizing a two-year-old adult male L. glama with increasing doses of B. atrox venom, followed by a VHH library construction and panning against partially purified hemorrhagic and myotoxic proteins. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format of the antibody completely inhibited the hemorrhagic activity of the venom fraction and was able to fully neutralize the hemorrhagic activity (Calderon et al., 2020; PMID: 32457735). |
Antibody First Published | Calderon et al. Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom Front Immunol. 2020 May 7:11:655. doi: 10.3389/fimmu.2020.00655. eCollection 2020. PMID:32457735 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
