Key features and details | |
Cat. No. | MABL-1805 |
Name | Anti-Hepatocyte growth factor mAbs |
Clone No. | AFD- t1E4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, IP, SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for HGF. It can bind to the two chains and to the single chain HGF. It recognizes an epitope located within the K4-SP region. |
Alternative Name | HGF; HPTA; Hepatopoietin-A; Scatter factor |
UniProt | P14210 |
Immunogen | The original antibody was generated by immunizing MRL/lpr mice with tcK2SP(Xa). |
Application Notes | The specificity of the original format of the antibody for HGF was confirmed by ELISA analysis. The authors investigated if the antibody has any effect on the HGF-Met signaling. Met activation was strongly inhibited by the antibody, with an IC50 value of ~0.15 μg/ml or 0.1nM. In particular, antibody caused dose dependent reduction of the HGF-induced phosphorylation levels of Met (Tyr1234/1235), Akt (Ser473), and ERK (Thr202 and Tyr204). The inhibitory action of the antibody was further evaluated by two cell-based assays. The effect of all antibody on the HGF-induced scattering of Madin-Darby canine kidney (MDCK) cells was evaluated, and confirmed that only it could strongly suppress the scattering behavior of the cells when added at 10 μg/ml. Furthermore, the antibody was able to inhibit the HGF-stimulated migration of human liver bile duct carcinoma cells with a similar concentration dependency as the inhibition of Met phosphorylation in EHMES-1 cells. |
Antibody First Published | Umitsu et al. Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies. Sci Rep. 2016 Sep 9;6:33149. PMID:27608665 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
