Anti-Hepcidin-25 mAbs

Cat. No.

MABL-1811

Name

Anti-Hepcidin-25 mAbs

Clone No.

AFD- OH4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human hepcidin-25 near   the N-terminus region and does not cross react with human hepcidin-20 or   human hepcidin-22. This antibody also does not cross react with mouse or rat   hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the   liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide   stabilized by 4 disulfide bonds that plays a major role in innate immunity   and iron homeostasis. It controls the delivery of iron to blood plasma from   intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and   from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating   the sole cellular iron exporter, ferroprotein, which delivers iron to plasma   from all iron-transporting cells.

Alternative Name

HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1

UniProt

P81172

Immunogen

The original antibody was generated by immunizing mice with immunized with a N-terminal hepcidin peptide 'DTHFPIC' conjugated with a five amino acid peptide linker (i.e., GPGPG) to an OVA   peptide (amino acids 323-336) sequence, i.e., full-length immunogen DTHFPICGPGPGISQAVHAAHAEINE.

Application Notes

The original mouse IgG1 antibody binds human   hepicidin-25 with a binding affinity of kd= 1.2 nM. The binding   characterization of this antibody towards human hepcidin was done using ELISA   (US8609817).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.