Key features and details | |
Cat. No. | MABL-1834 |
Name | Anti-Histone H3 mAbs |
Clone No. | AFD- 22F4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, SDS-PAGE, ELISA |
Species Reactivity | Bovine, Mouse |
Basic Information | |
Specificity | This antibody binds auto-antigen histone H3, and cross-reacts with bovine H3. |
Alternative Name | P84228; P84244; P68433 |
UniProt | |
Immunogen | This antibody was derived from spleen cells isolated from NC/Nga mice, which spontaneously develop severe atopic-like dermatitis and a strong associated serum IgE response under conventional conditions. |
Application Notes | The binding specificity of this antibody for histone H3 has been confirmed in SDS- PAGE, immunoblot and ELISA experiments (Matsubara et al, 2009). |
Antibody First Published | Matsubara et al. Serum and monoclonal immunoglobulin E antibodies from NC/Nga mice with severe atopic-like dermatitis recognize an auto-antigen, histone H3. Clin Exp Allergy. 2009 Apr;39(4):579-90 PMID:19226275 |
Note on publication | Describes the original generation of this antibody. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |