Key features and details | |
Cat. No. | MABL-1837 |
Name | Anti-Histone H4 mAbs |
Clone No. | AFD- 1A9D7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody specifically recognizes simultaneous acetylations at K5 and K8 of histone H4. Lysine acetylation of core histones is one of the major posttranslational modifications involved in the control of eukaryotic gene expression. |
Alternative Name | H4K5acK8ac; Histone H4 protein |
UniProt | P62805 |
Immunogen | The original antibody was generated by immunizing mice with Histone H4 protein with site- specific acetylations at K5 and K8. |
Application Notes | The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018; PMID: 30080437). |
Antibody First Published | Handoko et al. JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states. Epigenetics. 2018;13(4):410-431. PMID:30080437 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
