Key features and details | |
Cat. No. | MABL-1842 |
Name | Anti-HIV-1 surface protein gp120 mAbs |
Clone No. | AFD- 694-D |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IB, neutralization, ELISA |
Species Reactivity | Human immunodeficiency virus type 1 |
Basic Information | |
Specificity | This antibody is specific for the GRAF sequence of the gp120 V3 loop of HIV-1. |
Alternative Name | Surface protein gp120; Envelope glycoprotein gp160; Env polyprotein |
UniProt | P05877 |
Immunogen | The original antibody was generated by Epstein-Barr virus transformation of peripheral blood mononuclear cells from HIV-seropositive individuals and screening for reactivity against the rgp120IIIB peptide. |
Application Notes | The original version of this antibody (human IgG1) was characterized for its specificity and reactivity using ELISA and IB assays. In the ELISA, it reacted with the V3 of HIV strains MN, SF-2, HXB2, RF, WM52, NY5, SC, and CDC4 with an affinity (Kd) of 1.8 µM and 12.0 µM for SF-2 and HXB2, respectively. In an IB, this antibody reacted with gp120HTLV-IIIB. In in vitro neutralization assays, this antibody exhibited neutralizing activity against multiple HIV-1 strains, exhibiting broad reactivity (US5914109A). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
