Anti-HLA allele DQB1*0601 mAbs

Cat. No.

MABL-1844

Name

Anti-HLA allele DQB1*0601 mAbs

Clone No.

AFD- LG11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises peptides   corresponding to the tip of the MHC antigen-binding cavity (region 70 - 90)   of HLA allele DQB1*0601 (RTRAELDTVCRHNYEVAFRGI). It cross-reacts with peptide   DQB1*03/70–90, and the shorter peptide DQB1*06/70–79

Alternative Name

UniProt

Immunogen

This antibody was raised by immunising   BALB/c mice with unconjugated free synthetic DQB1 peptides (region 70–90).

Application Notes

This antibody blocks the myasthenia gravis  (MG)-associated DQ beta 1 allele, and is able to inhibit the proliferation of   T cells from MG patients (Ayyar & Atassi, 2017). A humanised scFv derived   from this antibody, which has been confirmed in ELISA analysis to recognise   the same epitope, is also able to block the proliferation of T cells from MG   patients typed DQB1*0601 (Ayyar & Atassi, 2017).

Antibody First   Published

Oshima et al. Suppression by mAbs against   DQB1 peptides of in vitro proliferation of AChR-specific T cells from   myasthenia gravis patients Autoimmunity, 2005. 38:2, 161-169 PMID:16040337

Note on publication

Describes the original generation of this   antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.