Anti-HMGB1 mAbs

Cat. No.

MABL-1873

Name

Anti-HMGB1 mAbs

Clone No.

AFD- 2G7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, WB, ELISA, IF

Species Reactivity

Rat

Basic Information

Specificity

This antibody is specific for amino acids 53-63 of HMGB1.

Alternative Name

Hmg-1; Hmg1; Amphoterin; Heparin-binding protein p30; High mobility group protein 1; High mobility group protein B1

UniProt

P63159

Immunogen

The original antibody was raised by immunizing   BALB/c mice with recombinant CBP-Rat HMGB1.

Application Notes

The original antibody was used for an ELISA   on recombinant rat HMGB1. This antibody was used for a western blot on   recombinant rat HMGB1. The epitope of this antibody was determined using   peptide binding. Mice were subjected to a cecal ligation and puncture, after   which this antibody was administered. It showed an increased survival rate in   these septic mice (US7632500B2). This antibody was used for   immunofluorescence on RAW 264.7 cells, to study intracellular HMGB1. This   antibody was used for an ELISPOT on RAW 264.7, C57 and HMC-12 cells (Wähämaa   et al., 2007; PMID:16980509). This antibody was administered to mice   subjected to ACLT, the antibody was able to protect cartilage (Aulin et al., 2020; PMID:31982563). This antibody was tested in two murine arthritis   models, it showed a decrease in general disease severity (Schierbeck et al.,   2011; PMID:21666956). 

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.