Key features and details | |
Cat. No. | MABL-1877 |
Name | Anti-homogalacturonan mAbs |
Clone No. | AFD- LM19 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, WB, ELISA, IF, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for unesterified homogalacturonan. Homogalacturonan is expressed prefominantly in the cell wall. |
Alternative Name | |
UniProt | |
Immunogen | The rat version of this antibody was raised by immunizing a wister rat with a polysaccharide. |
Application Notes | To characterize this antibody, an ELISA was performed on different pectins using the original rat version of this antibody. Furthermore, indirect immunofluorescence was performed on tobacco stem pith parenchyma using the rat version of this antibody (Verhertbruggen et al, 2009; pmid:19144326). To investigate the processing of pectin in fruit and vegetable tissue, an immunoblot was performed on pectin samples using the rat version of this antibody, Furthermore, raw and thermally treated broccoli pith parenchyma were stained for immunofluorescence using this antibody (Christiaens et al, 2011). Cell walls of Burlat and Samba sweet cherry cultivars were stained for immunofluorescence using the rat version of this antibody to study cracking in apple cultivars (Schumann et al, 2019; pmid:31365556). To determine the distribution of AGPs and pectic polysaccharides contained in apple fruit during senescence, immunocytochemistry was performed on different tissues from the cultivar Gala Must using the rat version of this antibody (Leszczuk et al, 2019; pmid:30007326). |
Antibody First Published | Verhertbruggen et al. An extended set of monoclonal antibodies to pectic homogalacturonan Carbohydr Res. 2009 Sep 28;344(14):1858-62. |
Note on publication | Antibodies againt saccharides were created and characterized. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
