Key features and details | |
Cat. No. | MABL-1907 |
Name | Anti-human insulin receptor mAbs |
Clone No. | AFD- HIRMAb |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, in vitro, in vivo, ELISA |
Species Reactivity | Human, Rhesus Monkey, Mouse |
Basic Information | |
Specificity | This antibody is specific for the endogenous human insulin receptor and binds an exofacial epitope within the 469–592 amino acid region of the receptor. This antibody cross-reacts with Rhesus monkey and mouse insulin receptors but does not cross the blood-brain barrier (BBB) in mice. |
Alternative Name | CD220; HHF5; IR; HIR; INSR; HINSR; human insulin receptor monoclonal antibody; 83-14 |
UniProt | P06213 |
Immunogen | This humanized antibody, based on the anti-insulin receptor alpha antibody clone 83-14 (Ab00137), was generated by grafting the complementarity-determining regions (CDRs) of the original murine IgG1 antibody onto the human B43 IgG1 heavy chain and the human REI kappa light chain frameworks. |
Application Notes | This humanized antibody clone, optimized for minimal immunogenicity in humans while retaining binding affinity to its target receptor, utilizes the BBB's endogenous insulin transport system to access the brain via receptor-mediated transcytosis without disrupting normal insulin transport (Boado, 2008; PMID: 19180267). This antibody was used to investigate binding and transport across the BBB and its application in enzyme replacement therapy for brain diseases (US7388079B2). Avid binding to human brain capillaries in vitro, comparable to the murine form, was demonstrated via a radioreceptor assay. It also crossed the Rhesus monkey’s BBB in vivo, specifically accumulating in areas of higher vascular density. Additionally, the light chain’s role in binding to the human insulin receptor was explored, revealing that variations in the light chain sequence minimally affect binding affinity, as tested in an ELISA, indicating flexibility in light chain design for humanized antibodies (US7388079B2, Boado et al., 2007; PMID: 16937408). |
Antibody First Published | Boado et al. Humanization of anti-human insulin receptor antibody for drug targeting across the human blood-brain barrier. Biotechnol Bioeng. 2007 Feb 1;96(2):381-91. doi: 10.1002/bit.21120. PMID:16937408 |
Note on publication | The original publication describes the humanization of a murine monoclonal antibody targeting the human insulin receptor to enable its use as a brain drug delivery system by facilitating transport across the human blood-brain barrier. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
