Anti-I-Ab mAbs

Cat. No.

MABL-1915

Name

Anti-I-Ab mAbs

Clone No.

AFD- AF6-120.1.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for the mouse Mouse I-Ab antigen.

Alternative Name

H-2 class II histocompatibility antigen, A-B   alpha chain; H2-Aa; Iaalpha

UniProt

P14434

Immunogen

The antibody was raised against the mouse   I-Ab antigen.

Application Notes

Cloned lines of functionally active murine T   lymphocytes derived from secondary mixed leukocyte cultures (MLC) were  immunostained with this antibody (Lorber et al, 1982; pmid:6804568). Cloned   lines of functionally active murine T lymphocytes derived from secondary   mixed leukocyte cultures (MLC) were used in flow cytometry using this   antibody (Lorber et al, 1982; pmid:6804568). Immunofluorescence was preformed   on splenocytes from NOD, NON and CTS mice using this antibody (Ikegami et al,   1988; pmid:3133269). Flow cytometry was preformed using this antibody on   TC-1. TC-1 is a malignant, immunogenic cell line of C57BL/6 mouse origin   expressing HPV16 E6/E7 antigens (Manning et al, 2008; pmid:17725605). Cells   from a p50-/- mouse were used in a flow cytometry ecperiment (Sha et al,   1995; pmid:7834752).

Antibody First   Published

Lorber et al. I-A ANTIGENS ON CLONED   ALLOREACTIVE MURINE T LYMPHOCYTES ARE ACQUIRED PASSIVELY J Immunol. 1982   Jun;128(6):2798-803. PMID:6804568

Note on publication

Cloned lines of alloreactive cytolytic and   amplifier T lymphocytes were examined for expression of I-A antigens.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.