Anti-IdeS-cleaved hinge of IgG1 mAbs

Cat. No.

MABL-1928

Name

Anti-IdeS-cleaved hinge of IgG1 mAbs

Clone No.

AFD- C2095

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ADCC assays, CDC assays, Depletion, ELISA

Species Reactivity

Human

Basic Information

Specificity

C2095 targets the IdeS‐cleaved hinge of human IgG1, but not intact IgG1 or   IgG1 cleaved by MMP-3 or GluV8. The antibody can also bind IdeS-cleaved hinge   of human IgG4 but not IdeS-cleaved hinge of IgG2.

Alternative Name

IdeS-cleaved hinge of IgG4

UniProt

Immunogen

This antibody was raised by immunising   rabbit with a KLH‐conjugated peptide   (CTAPPAPAPELLG).

Application Notes

ELISA demonstrated C2095 has robust binding   to IdeS-generated F(ab')2 fragments of human IgG1 and human IgG4 but does not   bind IdeS-generated F(ab')2 fragments of human IgG2. It also does not bind   the intact IgG1, IgG2 or IgG4 counterparts (Brezski et al, 2014). ADCC and   CDC assays showed C2095 is capable of restoring antibody-dependent   cell-mediated cytotoxicity and complement- dependent cytotoxicity against   WIL2-S cells to the otherwise inactive anti-CD20 IgG1 F(ab’)2 fragment. It   also reinstated ADCC against MDA-MB-231 cells to an anti-CD142 IgG1 F(ab’)2   fragment. This antibody was also capable of eliciting both CDC and ADCC to   IgG4 F(ab’)2 fragments (Brezski et al, 2014).

Antibody First   Published

Brezski et al, 2014. A monoclonal antibody against hinge-cleaved IgG restores effector function to   proteolytically-inactivated IgGs in vitro and in vivo. MAbs.   2014;6(5):1265-73 PMID:25517311

Note on publication

Describes the original generation of C2095   and its characterisation through ADCC and FCC assays.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.