Key features and details | |
Cat. No. | MABL-1954 |
Name | Anti-IGF1R mAbs |
Clone No. | AFD- IGFR 16-13 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, stimulatory |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically a conformation-dependent epitope in the alpha subunit (amino acids 62-184). It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays. |
Alternative Name | CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; 6 13 |
UniProt | P08069 |
Immunogen | This antibody was raised by immunising BALB/c mice with transfected mouse fibroblasts expressing high levels of human IGF1R (IGF1R/3T3). |
Application Notes | The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates IGF1R solubilized from IGF1R/3T3 cells and receptor-IGF1 complexes (Soos et al, 1992). When immobilised, this antibody has been shown to remove 90% of all soluble IGF1R in immunodepletion experiments (Soos et al, 1992). It has also been used to capture IGF1R N-terminal fragments as part of a ligand plate binding assay (Molina et al, 2000). This antibody strongly stimulates both IGF1 and insulin binding to IGF1R/3T3 cells, and potentiates insulin stimulation of DNA synthesis (Soos et al, 1992). |
Antibody First Published | Soos et al. A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity. J Biol Chem. 1992 Jun 25;267(18):12955-63. PMID:1377676 |
Note on publication | Describes the original generation of this antibody, and the characterisation of its specificity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
