Anti-IGF1R mAbs

Cat. No.

MABL-1959

Name

Anti-IGF1R mAbs

Clone No.

AFD- IGFR 24-60

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibitory, IP

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the human   insulin-like growth factor 1 receptor (IGF1R). It recognises the   extracellular portion of the receptor, specifically an epitope in the   cysteine rich domain of the alpha subunit, between amino acids 184 and 283.   It does not cross-react with rat or mouse IGF1R or human insulin receptors in   co-precipitation or immunodepletion assays.

Alternative Name

IGF1 receptor; Insulin-like growth factor 1   receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I   receptor; CD221; 24 60

UniProt

P08069

Immunogen

This antibody was raised by immunising   BALB/c mice with transfected mouse fibroblasts expressing high levels of   human IGF1R (IGF1R/3T3).

Application Notes

The ability of this antibody to bind human   IGF1R has been confirmed in IGF1 binding assays using both   IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al,   1992). This antibody efficiently precipitates human IGF1R solubilised from   IGF1R/3T3 cells (Soos et al, 1992) and human placental membranes (Bailyes et   al, 1997), and has been used in immunoprecipitation analysis of 293 cells   transfected with IGF1R or chimeric IGF1R-insulin receptor (IR) constructs   (Schumacher et al, 1993). When immobilised, this antibody has been shown to   remove essentially all soluble, IGF1R/3T3-derived IGF1R (Soos et al, 1992)   and human placental membrane-derived IGF1R (Soos et al, 1993) in   immunodepletion experiments. This antibody strongly inhibits IGF1 binding to   its receptor (Soos et al, 1992), and has been shown to suppress IGF1-mediated   stimulation of skin fibroblast proliferation (Okubo et al 2003).

Antibody First   Published

Soos et al. A panel of monoclonal antibodies   for the type I insulin-like growth factor receptor. Epitope mapping, effects   on ligand binding, and biological activity. J Biol Chem. 1992 Jun   25;267(18):12955-63. PMID:1377676

Note on publication

Describes the original generation of this   antibody, and the characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.