Anti-IgG1 Fc mAbs

Cat. No.

MABL-1977

Name

Anti-IgG1 Fc mAbs

Clone No.

AFD- TP1107

From

Recombinant Antibody

Isotype

Engineer antibody

Application

dot blot assay, ICC, WB, IF

Species Reactivity

Human (weak), Rat, Mouse

Basic Information

Specificity

This antibody recognises Fc fragment of the IgG1.

Alternative Name

immunoglobulin G1 fragment crystallizable region; immunoglobulin G1 Fc region; immunoglobulin G1

UniProt

Immunogen

This antibody was raised by immunising alpacas   with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody   immune libraries and the selection of antigen-specific nanobodies by phage   display from these libraries were performed.

Application Notes

Anti-IgG nanobodies can often perform more   efficiently than polyclonal secondary antibodies in various experimental   assays. For instance, this nanobody conjugated with a maleimide- activated   HRP was used to detect mouse IgG1 via ECL Western blot and it considerably   outperformed a polyclonal secondary antibody–HRP conjugate (Pleiner et al.,   2018). Similarly, this nanobody conjugated with the infrared fluorophore   IRDye 800 performed equally or better than a polyclonal anti–mouse IgG   secondary antibody in Western blotting on HeLa cell lysate (Pleiner et al.,   2018). TP1107 nanobody can also be used as an efficient high-affinity reagent   for immunofluorescence. It was utilised in conjugation with two to three  fluorophores in detection of mouse monoclonal IgG1 for the indirect   immunostaining of HeLa cells where it performed exceptionally well as for a   non-polyclonal antibody reaching similar signal strength to polyclonal agents   when it was combined with another anti-IgG1 nanobody (Pleiner et al., 2018).  

Antibody First   Published

Pleiner et al. A toolbox of anti–mouse and   anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar   5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20.   PMID:29263082

Note on publication

The article describes the generation and characterisation of this nanobody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.