Key features and details | |
Cat. No. | MABL-1993 |
Name | Anti-IgG2a Fc mAbs |
Clone No. | AFD- TP1129 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | dot blot assay, ICC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognises Fc region of mouse IgG2a. |
Alternative Name | immunoglobulin G2a fragment crystallizable region |
UniProt | |
Immunogen | This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed. |
Application Notes | TP1129 can be used as an efficient secondary nanobody in the indirect immunofluorescence. It was utilised to detect IgG2a primary antibodies alongside another nanobody giving strong, clean signal without any background staining (Pleiner et al., 2018). |
Antibody First Published | Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3)：1143-1154. doi： 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID：29263082 |
Note on publication | The article describes the generation and characterisation of this nanobody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
