Key features and details | |
Cat. No. | MABL-2003 |
Name | Anti-IgM mAbs |
Clone No. | AFD-1.14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | affinity chromatography, IP, RIP, ELISA, FC, IF |
Species Reactivity | Trout |
Basic Information | |
Specificity | This antibody is specific for trout IgM. It reacts with the IgM heavy chain (Hedfors et al, 2012, pmid:23009920). It is not directed against nonprotein antigenic parts of the IgM, which is shown by the failure of IgM glycopeptides to inhibit precipitation (Deluca et al, 1983, pmid:6347695). 1.14 is crossreactive with atlantic salmon IgM (Hedfors et al, 2012, pmid:23009920). The antibody targets either IgM-A of IgM-B (Hedfors et al, 2012, pmid:23009920). It has been shown that this antibody does not show crossreactivity with catfish IgM (Moulana et al, 2008, pmid:18676026). |
Alternative Name | Trout immunoglobulin M; immunoglobulin M |
UniProt | |
Immunogen | This antibody was raised by immunizing female BALB/c mice with purified trout IgM. |
Application Notes | This antibody has been used for affinity chromatography with the protein A (Deluca et al, 1983, pmid:6347695). The antibody was shown to be effective in flow cytometry application (Deluca et al, 1983, pmid:6347695). It was demonstrated that this antibody can be utilized in the immunofluorescence staining of trout head kidney, spleen and thymus cells (Deluca et al, 1983, pmid:6347695). Furthermore, it has been shown that this antibody can be used in the immunoprecepitation of trout IgM (Deluca et al, 1983, pmid:6347695). It is used in western blots as a negative against catfish IgL σ (Edholm et al, 2010, pmid:20149930) and MHC class IIβ (Moulana et al, 2008, pmid:18676026). Finally, 1.14 antibody can be used to detect trout antibody in an ELISA (Costa et al, 2011, pmid: 21641928). |
Antibody First Published | Dominick DeLuca, Melanie Wilson and Gregory W. Warr Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM* Eur. J. Immunol 13: 546-551. PMID:6347695 |
Note on publication | It describes the generation of the antibody and its use in research on the trout immune system. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
