Anti-IL-13 mAbs

Cat. No.

MABL-2027

Name

Anti-IL-13 mAbs

Clone No.

AFD- 6A1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, neutralization, SPR, ELISA

Species Reactivity

Human, Cynomolgus Monkey

Basic Information

Specificity

This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13.

Alternative Name

IL13; Interleukin-13; P600

UniProt

P35225

Immunogen

The original antibody was generate by   immunizing SJL mice with E. Coli-produced recombinant human IL-13.

Application Notes

The original format of this antibody (mouse   IgG1) showed binding activity against E.coli-expressed recombinant human   IL-13 in a sandwich ELISA with an EC50 of 0.049 and 0.039 µg/ml for human and   cynomolgus IL-13. The antibody also exhibited potent binding and   neutralization activities against various forms of IL-13. In inhibition   assays, the antibody showed IC50 values of 0.165 µg/ml for blocking human   IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2.   Moreover, it demonstrated strong neutralization capabilities with ND50   (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for   cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1   cell proliferation (where the ND50 value is the concentration of monoclonal   antibody required to reduce TF-1 cell proliferation by 50%, in response to a   set concentration of IL-13).

Antibody First   Published

Note on publication

The original publication describes the   generation and characterization of an anti- human IL-13 antibody and its   derivatives, including its humanization.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.