Key features and details | |
Cat. No. | MABL-2049 |
Name | Anti-IL-1A mAbs |
Clone No. | AFD- 9H10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds mouse Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. |
Alternative Name | IL1A; IL-1α; Interleukin-1 alpha; IL-1 alpha |
UniProt | P01582 |
Immunogen | The original antibody was generated by immunizing IL-1α/β double KO mice with mouse IL-1 alpha. |
Application Notes | The original mouse IgG antibody binds mouse IL-1A with a binding affinity of Kd= 0.17 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.00E−10M. This antibody was used in combination with 10G11 (anti- mouse IL-1B) antibody for the generation of anti-mouse IL-1α/β DVD-Ig molecule (US7612181). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
