Key features and details | |
Cat. No. | MABL-2059 |
Name | Anti-IL-2 mAbs |
Clone No. | AFD- LNKB-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, FACS, Latex agglutination, WB, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human IL-2 at the 59-72 site of the IL-2 sequence. |
Alternative Name | Interleukin 2; LNKB2 |
UniProt | P60568 |
Immunogen | |
Application Notes | This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ≈ 3 × 108 M-1) (Lunev et al., 1990; PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993; PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000; PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the α-helical character of the epitope fragment (Afonin et al., 2001; PMID: 11468348). |
Antibody First Published | Lunev et al. Latex-agglutination analysis of human recombinant interleukin- 2 with monoclonal antibodies Biomed Sci. 1990 Jan;1(1):68-72 PMID:1718463 |
Note on publication | The original publication describes the generation and characterization of monoclonal antibodies to human interleukin-2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
