Anti-IL-34 mAbs

Cat. No.

MABL-2072

Name

Anti-IL-34 mAbs

Clone No.

AFD- FAb1.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, crystallization, neutralizing

Species Reactivity

Human, Mouse

Basic Information

Specificity

The antibody is specific for human and   murine IL-34. The antibody recognizes a largely continuous area at the   junction of two IL-34 protomers. The epitope overlaps largely with binding   epitopes responsible for CSF-1R binding.

Alternative Name

Interleukin-34; YW404.33.56

UniProt

Q6ZMJ4

Immunogen

The original antibody was generated by   panning a Fab library against IL-34.

Application Notes

The crystal structure of the Fab fragment of   the antibody in complex with human IL-34 was determined. The original   antibody was reformatted as an IgG molecules and the binding affinity of both   formats measured by BLI experiments (Kd= 17 nM and 120 pM respectively). The   antibody blocked the biological activity of IL-34 in human monocyte   viability. The antibody neutralized IL-34 in a proliferation assays (Ma et   al., 2012; PMID: 22483114).

Antibody First   Published

Ma et al. Structural basis for the dual   recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure. 2012   Apr 4;20(4):676-87. PMID:22483114

Note on publication

The original paper shows the structure of   IL-34 in complex with the Fab fragment of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.