Key features and details | |
Cat. No. | MABL-2137 |
Name | Anti-Inosine5'-monophosphate Dehydrogenase (IMPDH) mAbs |
Clone No. | AFD- 1D6-E3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB |
Species Reactivity | Human, Rodent |
Basic Information | |
Specificity | This antibody is specific for the IMPDH protein in both humans and rodents. IMPDH catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. |
Alternative Name | Inosine-5'-monophosphate dehydrogenase 2; IMPDH2; IMP dehydrogenase 2; IMPD 2; IMPDH 2; IMPDH-II |
UniProt | P12269 |
Immunogen | The antibody was generated by immunizing a mouse with the purified Chinese hamster IMPDH protein. |
Application Notes | This antibody was used for a western blot on proteins from E.coli cells that were made to express IMPDH (Hager et al, 1995; pmid:7763314). HL-205 cells were used in a peroxidase-based western blot with this antibody (Glesne et al, 1991; pmid:7763314). HL-205 cells were used in a peroxidase-based western blot with this antibody (Glesne et al, 1991; pmid:1717828). |
Antibody First Published | Glesne et al. Regulation of IMP Dehydrogenase Gene Expression by Its End Products, Guanine Nucleotides MOLECULAR AND CELLULAR BIOLOGY, Nov. 1991, p. 5417-5425 PMID:1717828 |
Note on publication | the regulation of IMP dehydrogenase (IMPDH), the rate-limiting enzyme of guanine nucleotide biosynthesis was studied, the effects of nucleosides, nucleotides, nucleotide analogs, or the IMPDH inhibitor mycophenolic acid (MPA) on the steady-state levels of IMPDH mRNA were examined. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
