Anti-JAML mAbs

Cat. No.

MABL-2175

Name

Anti-JAML mAbs

Clone No.

AFD- HL4E10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, FACS, IP, SPR, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

HL4E10 was found to be specific for JAML, as   this protein was identified as the ligand for the mAb from   immunoprecipitation (Witherden et al, 2010). Biochemical binding studies showed that HL4E10 bound the JAML D2 Ig domain, which was corroborated with a   recent crystal structure of the JAML ECD and a HL4E10 Fab fragment (Verdino   et al, 2011). HL4E10 has an affinity for JAML of 8 nM, characterised by SPR.

Alternative Name

MICA1; Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1

UniProt

Q86YT9

Immunogen

An Armenian hamster was immunised with the   7-17 dendritic epidermal T cell line. Splenocytes were obtained from   immunised hamsters and fused with the X63Ag8.653 myeloma cell line to   generate a hybridoma.

Application Notes

HL4E10 has been used to detect JAML by both IP and FC (Witherden et al, 2010). IF labelling showed that JAML colocalises   with the T cell receptor.

Antibody First   Published

Witherden et al. The junctional adhesion   molecule JAML is a costimulatory receptor for epithelial gammadelta T cell   activation. Science. 2010 Sep 3;329(5996):1205-10. PMID:20813954

Note on publication

Describes the characterisation of JAML as a   gammadelta T cell surface marker. mAb HL4E10 was produced and characterised   herein as a JAML activator.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.