Key features and details | |
Cat. No. | MABL-2204 |
Name | Anti-Kit mAbs |
Clone No. | AFD- 79D |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, inhibition, IP, SPR, WB |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for KIT, and it binds to the membrane-proximal Ig-like D4 of KIT. The receptor tyrosine kinase KIT is a transmembrane protein that plays crucial roles in mediating diverse cellular processes, including cell differentiation, proliferation, and cell survival, among other activities. |
Alternative Name | Mast/stem cell growth factor receptor Kit (EC:2.7.10.1); SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117 |
UniProt | P10721 |
Immunogen | Phage pools consisting of a phage-displayed synthetic antibody library (library F) were cycled through five rounds of selections by using KITD4-5. |
Application Notes | The crystal structure of the Fab in complex with KIT D4D5 fragment was determined. Surface plasmon resonance analysis was employed to quantitatively characterize the kinetics and dissociation constants of the Fab (Kd = 6.4 pM). 3T3 cells expressing WT KIT receptor were incubated with the IgG and Fab versions of the antibody following stimulation with SCF. Lysates of unstimulated or SCF- stimulated cells were subjected to immunoprecipitation with the IgG and Fab followed by SDS/PAGE and immunoblotting with IgG and Fab. SCF-stimulated autophosphorylation of KIT was strongly inhibited upon binding of both fragments (5 nM for Fab and 1 nM for IgG). The IgG and Fab fragments inhibited the proliferation of KIT-Dependent Ba/F3 cells. The Fab fragment showed inhibition of cell growth at 5 nM, while the IgG fragment at 10 nM (Reshetnyak et al. 2013; PMID: 24127596). |
Antibody First Published | Reshetnyak et al. Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proc Natl Acad Sci U S A. 2013 Oct 29; 110(44): 17832–17837. PMID:24127596 |
Note on publication | The paper describes the generation and characterization of a panel of inhibitory KIT antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
