Key features and details | |
Cat. No. | MABL-2216 |
Name | Anti-LAG-3 mAbs |
Clone No. | AFD- Ab2317 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, in vivo, SPR |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human LAG-3. The antibody does not detect cynomolgus LAG-3 or murine LAG-3. |
Alternative Name | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
UniProt | P18627 |
Immunogen | |
Application Notes | The antibody is a humanized version of mab23. The antibody had an activity EC50 of 0.34 μg/ml for activating human T cells to release IL-2. Further, the antibody activates human DC cell to stimulate human T cell activity (EC50 of 0.21nM), as shown by MLR assay. The humanized antibody efficacy in vivo was evaluated of the present invention. Tumors treated in mice showed an inhibition in tumor growth when treated with a combination of the antibody and PD-1 antibody. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.2 nM). The antibody showed a good blocking activity. The antibody was used in combination with an anti PD-1 antibody (Nivo or Pem) into a bispecific antibody (CN112079925). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
