Anti-loggerhead IgY heavy chain mAbs

Cat. No.

MABL-2272

Name

Anti-loggerhead IgY heavy chain mAbs

Clone No.

AFD- LH-12-4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Sea turtles

Basic Information

Specificity

Recognizes all sea turtle IgY heavy chains,   except for leather back sea turtles.

Alternative Name

immunoglobulin Y; HC; VH

UniProt

Immunogen

This antibody was raised by immunizing Balb/c mice with sea turtle IgY pirified using immunoglobulin-binding protein-G

Application Notes

To determine the reactivity of this antibody   it was immunoblotted with purified IgY (Rodgers and Rice, 2018;   pmid:29787778). This antibody was used as a part of an ELISA created for   quantifying bacteria-specific IgY titers in individual turtles (Rodgers and   Rice, 2018; pmid:29787778).

Antibody First   Published

Rodgers and Rice Monoclonal antibodies   against loggerhead sea turtle, Caretta caretta, IgY isoforms reveal   differential contributions to antibody titers and relatedness among other sea   turtles Dev Comp Immunol. 2018 Oct;87:12-15. PMID:29787778

Note on publication

mAb LH12 was developed against the 66 kDa   heavy chain of IgY, mAb LH1 was developed against the truncated heavy chain   of approximately 37 kDA, and mAb LH9 was developed against the 23 kDa light   chains.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.